EasyFlow: An Open-Source, User-Friendly Flow Cytometry Analyzer

EasyFlow Cytometry Analyzer
Application
🧬
EasyFlow is a free and open-source software for flow cytometry analysis, making it accessible for users without coding experience and providing flexibility for advanced users.
Contributors
👥
Developed by Yitong Ma and Yaron Antebi, EasyFlow provides open-source analysis tools to the cytometry community.
Contact
✉️
Free Download
💻
Access EasyFlow from GitHub – EasyFlow or EasyFlowQ Website
Progress
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EasyFlow is fully functional with core features like gating and plotting, and continues to evolve with community contributions.

EasyFlow is an innovative software tool designed to simplify flow cytometry data analysis. Flow cytometry is a method that measures characteristics of single cells, like fluorescence levels, and is widely used in fields such as immunology and microbiology. While many existing analysis tools require coding knowledge or are costly, EasyFlow is open-source, free to use, and doesn’t require coding experience. Available in both Matlab and Python versions, it offers easy access for users on Windows, MacOS, and Linux.

How It Works: EasyFlow is designed to analyze flow cytometry data, making it straightforward to view, gate, and analyze single-cell data. Users can create standard charts, such as histograms and scatter plots, directly through the software’s visual interface. For advanced users, EasyFlow offers options to export data for further analysis in Matlab or as .csv files for use in Excel or Python.

Main Features:

  • Cross-Platform Compatibility: Works on Windows, MacOS, and Linux.
  • No Coding Required: Easy for beginners, while offering customization options for advanced users.
  • Visualization Tools: Includes interactive plotting for data exploration and analysis.
  • Export Options: Users can export raw data for in-depth analysis.

Progress Updates:

  • Basic functions: Complete (e.g., loading FCS files, gating, plotting)
  • Standalone Python version (EasyFlowQ): Provides a fully independent package for users without Matlab or Python installations.

Community Collaboration: The project is hosted on GitHub, allowing users to contribute, suggest features, and report bugs. With its open-source nature, EasyFlow encourages community-driven improvements and is well-suited for both academic and research settings.

For more details or to download EasyFlow, visit the EasyFlow GitHub page or EasyFlowQ website.

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