Application
The Pi-seq platform is ideal for precise liquid handling in microfluidics, supporting applications like single-cell RNA sequencing.
Contributors
Developed by researchers at Philipps University of Marburg, Germany, in collaboration with experts in synthetic microbiology and biostatistics.
Contact
Free Download
Access the full design and source files at OSF Repository.
Progress
Pi-seq is fully functional and actively supported by a growing community of researchers and engineers.
Resources
In a new milestone for open-source hardware, the Pi-seq is introduced as an affordable, customizable, and modular multi-channel syringe pump for microfluidics. The project, published in HardwareX, is designed to provide an accessible solution for complex biological experiments like single-cell RNA sequencing. Unlike expensive commercial devices, Pi-seq utilizes widely available components, including a Raspberry Pi and 3D-printed parts, making it a cost-effective and highly adaptable platform.
Key Features:
- Modular Design: Allows users to customize the pump channels based on their requirements.
- Affordable: Complete setup costs less than €600.
- Temperature Control: Integrated Peltier-based system for precise temperature regulation.
- Mixing Capability: Equipped with a mixer to prevent sedimentation of cells.
- Open-Source: Licensed under GPL 3.0 (software) and CC-BY-NC (hardware).
- Compatibility: Seamlessly integrates with common microfluidic chips and microscopes.
- User-Friendly Interface: Controlled via a Raspberry Pi touchscreen, eliminating the need for additional boards like Arduino.
The Pi-seq is an open-source innovation that addresses the limitations of existing commercial microfluidics solutions, providing a versatile and reliable tool for a wide range of life science applications.
