This paper introduces a portable and user-friendly microfluidic liquid handling automation platform specifically designed for immunoassays. The system eliminates the need for bulky external components and complicated setups, facilitating plug-and-play operation. By utilizing advanced microfluidic technology, this device enhances the efficiency and accuracy of immunoassays, which are critical in various clinical and research applications.
Key Features
- Portable Design: The device has a compact footprint of 16.5 cm x 11.5 cm x 7 cm and weighs 801 grams, making it easy to transport and use in various settings.
- Plug-and-Play Operation: The system features a ‘clamshell-style’ cartridge that allows users to easily load and run assays without the need for extensive setup.
- Automated Liquid Handling: Equipped with a miniature electro-pneumatic controller, it can perform multi-reagent switching, precise metering, and timing control.
- Battery-Powered: A rechargeable battery allows for continuous operation for up to 42 hours.
- Versatile Applications: Ideal for point-of-care diagnostics, molecular diagnostics, and other applications that require precise liquid manipulation.
Specifications
| Specification | Details |
|---|---|
| Device Name | Handheld Plug-and-Play Microfluidic Liquid Handling Platform |
| Cost | $1,395 |
| Dimensions | 16.5 cm x 11.5 cm x 7 cm |
| Weight | 801 g (including battery) |
| Power Source | Rechargeable 12V battery |
| Operating Time | Up to 42 hours on a full charge |
| Open Source License | GPLv3 |
| Source File Repository | Design Files |
Build Instructions
- PCB Assembly: Solder the electronic components to the printed circuit board (PCB).
- 3D Printing: Print necessary components using specified materials.
- Assembly: Follow detailed instructions to assemble the cartridge and manifold systems.
- Connection: Wire the electronic components and connect the pneumatic tubing as per the design schematic.
Operation Instructions
- Power the System: Connect the rechargeable battery and turn on the device.
- Load the Cartridge: Insert the pre-loaded cartridge into the device.
- Run Assays: Use the interface to select and execute the desired assay protocol automatically.
Validation
The platform demonstrated a limit of detection of 31.1 ng/mL in a SARS-CoV-2 spike antibody immunoassay, showcasing performance comparable to established enzyme-linked immunosorbent assays (ELISA).
This handheld microfluidic platform provides an efficient, user-friendly solution for liquid handling in immunoassays, supporting a wide range of applications in point-of-care settings and research laboratories. Its design addresses common limitations in existing systems, enhancing accessibility and ease of use.
